GeneTex
United States (US)

UQCRC1 antibody [N1N3]

KO/KD
GTX101862_39568_20161103_WB_shRNA_watermark_19011506_420.jpg
GTX101862_39568_20141205_IHC_M_19011506_108.jpg
GTX101862_39568_20141205_IHC_M_2_19011506_844.jpg
GTX101862_39568_WB_M_brain_19011506_783.jpg

Cat No. GTX101862

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , Mouse brain

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquinol-Cytochrome C Reductase Core Protein 1,D3S3191,Qcr1,Uqcr1,Uqcrc1

Cellular Localization

Mitochondrion inner membrane

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.

Database

Research Area

DATA IMAGES
GTX101862_39568_20161103_WB_shRNA_watermark_19011506_420.jpg

GTX101862 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [N1N3] (GTX101862) diluted at 1:500.

GTX101862_39568_20141205_IHC_M_19011506_108.jpg

GTX101862 IHC-P Image

UQCRC1 antibody [N1N3] detects UQCRC1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
UQCRC1 antibody [N1N3] (GTX101862) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101862_39568_20141205_IHC_M_2_19011506_844.jpg

GTX101862 IHC-P Image

UQCRC1 antibody [N1N3] detects UQCRC1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
UQCRC1 antibody [N1N3] (GTX101862) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101862_39568_WB_M_brain_19011506_783.jpg

GTX101862 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101862 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169