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UQCRC1 antibody [N1N3]

KO/KD
Anti-UQCRC1 antibody [N1N3] used in Western Blot (WB). GTX101862
Anti-UQCRC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101862
Anti-UQCRC1 antibody [N1N3] used in Western Blot (WB). GTX101862
Anti-UQCRC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101862

Cat. No. GTX101862

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UQCRC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Mouse brain , 293T

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241430

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquinol-cytochrome c reductase core protein 1 , D3S3191 , QCR1 , UQCR1

Cellular Localization

Mitochondrion inner membrane

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.

Database

Research Area

DATA IMAGES

Anti-UQCRC1 antibody [N1N3] used in Western Blot (WB). GTX101862

GTX101862 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [N1N3] (GTX101862) diluted at 1:500.

Anti-UQCRC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101862

GTX101862 IHC-P Image

UQCRC1 antibody [N1N3] detects UQCRC1 protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
UQCRC1 antibody [N1N3] (GTX101862) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UQCRC1 antibody [N1N3] used in Western Blot (WB). GTX101862

GTX101862 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101862 diluted at 1:1000

Anti-UQCRC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101862

GTX101862 IHC-P Image

UQCRC1 antibody [N1N3] detects UQCRC1 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
UQCRC1 antibody [N1N3] (GTX101862) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169