APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:20000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , Huh7 , Hep3B
Predict Reactivity
Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11178216
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquinol-cytochrome c reductase core protein 1 , D3S3191 , QCR1 , UQCR1
Cellular Localization
Mitochondrion inner membrane
Background
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
Database
Research Area
DATA IMAGES
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GTX101896 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody (GTX101896) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101896 WB Image
UQCRC1 antibody detects UQCRC1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
10% SDS-PAGE
UQCRC1 antibody (GTX101896) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101896 ICC/IF Image
NYREN18 antibody [N1N3] detects NYREN18 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: NYREN18 protein stained by NYREN18 antibody [N1N3] (GTX101896) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX101896 WB Image
UQCRC1 antibody detects UQCRC1 protein by western blot analysis.
A Huh7 whole cell lysate/extract
B Hep3B whole cell lysate/extract
C HepG2 whole cell lysate/extract
10% SDS-PAGE
UQCRC1 antibody (GTX101896) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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