GeneTex
United States (US)

UQCRC2 antibody

Comparison
GTX114873_40646_20180126_WB_competitor_watermark_18121410_438.jpg
GTX114873_40646_20180119_WB_M_R_18121410_704.jpg
GTX114873_40646_IHC_18121410_115.jpg
GTX114873_40646_IP_18121410_128.jpg
GTX114873_40646_IFA_18121410_223.jpg

Cat No. GTX114873

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , mouse brain , rat brain

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UQCRC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquinol-Cytochrome C Reductase Core Protein 2,Mc3Dn5,Qcr2,Uqcr2,Uqcrc2

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.

Database

Research Area

DATA IMAGES
GTX114873_40646_20180126_WB_competitor_watermark_18121410_438.jpg

GTX114873 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000 and competitor's antibody (14742-1-AP) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX114873_40646_20180119_WB_M_R_18121410_704.jpg

GTX114873 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114873_40646_IHC_18121410_115.jpg

GTX114873 IHC-P Image

UQCRC2 antibody detects UQCRC2 protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
UQCRC2 antibody (GTX114873) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114873_40646_IP_18121410_128.jpg

GTX114873 IP Image

UQCRC2 antibody immunoprecipitates UQCRC2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of UQCRC2 protein by 4 μg UQCRC2 antibody (GTX114873)
10 % SDS-PAGE
The immunoprecipitated UQCRC2 protein was detected by UQCRC2 antibody (GTX114873) diluted at 1:5000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX114873_40646_IFA_18121410_223.jpg

GTX114873 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UQCRC2(GTX114873) antibody at 1:500 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169