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UQCRC2 antibody

Comparison Orthogonal
Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873
Anti-UQCRC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114873
Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873
Anti-UQCRC2 antibody used in Immunoprecipitation (IP). GTX114873
Anti-UQCRC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114873

Cat. No. GTX114873

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, PLA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all UQCRC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

mouse brain , rat brain , Jurkat , Raji , NCI-H929

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UQCRC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164243

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquinol-cytochrome c reductase core protein 2 , MC3DN5 , QCR2 , UQCR2

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.

Database

Research Area

DATA IMAGES

Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873

GTX114873 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000 and competitor's antibody (14742-1-AP) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-UQCRC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114873

GTX114873 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UQCRC2(GTX114873) antibody at 1:500 dilution.

Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873

GTX114873 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UQCRC2 antibody used in Immunoprecipitation (IP). GTX114873

GTX114873 IP Image

UQCRC2 antibody immunoprecipitates UQCRC2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of UQCRC2 protein by 4 μg UQCRC2 antibody (GTX114873)
10 % SDS-PAGE
The immunoprecipitated UQCRC2 protein was detected by UQCRC2 antibody (GTX114873) diluted at 1:5000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-UQCRC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114873

GTX114873 IHC-P Image

UQCRC2 antibody detects UQCRC2 protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
UQCRC2 antibody (GTX114873) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lee H et al. Nat Commun 2023; 14 (1) : 6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Matassa DS et al. Cancer Cell Int 2022; 22 (1) : 402 Regulation of mitochondrial complex III activity and assembly by TRAP1 in cancer cells.
Application : WB, PLA
Reactivity : Human
Mingming Zhao et al. Nat Commun 2022; 13 (1) : 1757 Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Lee H et al. J Biol Chem 2020; 295 (19) : 6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
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REVIEW

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Application Reference
Lee H et al. Nat Commun 2023; 14 (1):6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Avolio R et al. Genome Res 2023; 33 (8):1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Matassa DS et al. Cancer Cell Int 2022; 22 (1):402 Regulation of mitochondrial complex III activity and assembly by TRAP1 in cancer cells.
Application : WB, PLA
Reactivity : Human
Mingming Zhao et al. Nat Commun 2022; 13 (1):1757 Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Lee H et al. J Biol Chem 2020; 295 (19):6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Imami K et al. Mol Cell 2018; 72 (1):84-98.e9 Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis.
Application : WB
Reactivity : Human
Takahashi K et al. Exp Gerontol 2017; 98:217-223 Optic atrophy 1 mediates coenzyme Q-responsive regulation of respiratory complex IV activity in brain mitochondria.
Application : IP
Reactivity : Mouse
Lee H et al. J Biol Chem 2017; (Epub) The Short Variant of the Mitochondrial Dynamin OPA1 Maintains Mitochondrial Energetics and Cristae Structure.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169