GeneTex
United States (US)

USF2 antibody

Comparison
GTX129239_41661_20180314_ICC_IF_18121410_153.jpg
GTX129239_41661_20161110_WB_competitor_watermark_18121410_183.jpg
GTX129239_41661_20160908_WB_M_18121410_157.jpg

Cat No. GTX129239

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FIP antibody , USF2 antibody , USF2 antibody , bHLHb12 antibody , upstream transcription factor 2, c-fos interacting antibody

Cellular Localization

Nucleus

Background

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX129239_41661_20180314_ICC_IF_18121410_153.jpg

GTX129239 ICC/IF Image

USF2 antibody detects USF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USF2 stained by USF2 antibody (GTX129239) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

GTX129239_41661_20161110_WB_competitor_watermark_18121410_183.jpg

GTX129239 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with USF2 antibody (GTX129239) diluted at 1:500 and competitor's antibody (SC-862) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX129239_41661_20160908_WB_M_18121410_157.jpg

GTX129239 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with USF2 antibody (GTX129239) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169