GeneTex
United States (US)

USP14 antibody

KO/KD
GTX115186_40254_20180330_WB_KO_watremark_18121410_412.jpg
GTX115186_40254_IHC_18121410_434.jpg
GTX115186_40254_WB_M_brain_18121410_214.jpg
GTX115186_40254_20170511_WB_18121410_808.jpg
GTX115186_WB_18121410_669.jpg
GTX115186_40254_WB_R_brain_18121410_365.jpg

Cat No. GTX115186

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HeLa , A431 , Jurkat , mouse brain , rat brain , U87-MG , SK-N-SH , IMR32 , SK-N-AS , *K562

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human USP14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Specific Peptidase 14,Tgt,Usp14

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115186_40254_20180330_WB_KO_watremark_18121410_412.jpg

GTX115186 WB Image

KO/KD

Wild-type (WT) and USP14 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115186) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX115186_40254_IHC_18121410_434.jpg

GTX115186 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using USP14(GTX115186) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115186_40254_WB_M_brain_18121410_214.jpg

GTX115186 WB Image

USP14 antibody detects USP14 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
7.5 % SDS-PAGE
USP14 antibody (GTX115186) dilution: 1:1000

GTX115186_40254_20170511_WB_18121410_808.jpg

GTX115186 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115186) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115186_WB_18121410_669.jpg

GTX115186 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: JurKat
7.5% SDS PAGE
GTX115186 diluted at 1:1000

GTX115186_40254_WB_R_brain_18121410_365.jpg

GTX115186 WB Image

USP14 antibody detects USP14 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
USP14 antibody (GTX115186) dilution: 1:1000

REFERENCE
REVIEW

USP14 antibody Cat No. GTX115186

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-May-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : K562 cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 10Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169