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USP14 antibody

KO/KD
Anti-USP14 antibody used in Western Blot (WB). GTX115186
Anti-USP14 antibody used in Western Blot (WB). GTX115186
Anti-USP14 antibody used in IHC (Paraffin sections) (IHC-P). GTX115186
Anti-USP14 antibody used in Western Blot (WB). GTX115186
Anti-USP14 antibody used in Western Blot (WB). GTX115186
Anti-USP14 antibody used in Western Blot (WB). GTX115186

Cat. No. GTX115186

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all USP14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

mouse brain , A431 , Jurkat , rat brain , U87-MG , SK-N-SH , IMR32 , SK-N-AS , HeLa

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human USP14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620301

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 14 , TGT

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-USP14 antibody used in Western Blot (WB). GTX115186

GTX115186 WB Image

KO/KD

Wild-type (WT) and USP14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115186) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP14 antibody used in Western Blot (WB). GTX115186

GTX115186 WB Image

USP14 antibody detects USP14 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5 % SDS-PAGE
USP14 antibody (GTX115186) dilution: 1:1000

Anti-USP14 antibody used in IHC (Paraffin sections) (IHC-P). GTX115186

GTX115186 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using USP14(GTX115186) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP14 antibody used in Western Blot (WB). GTX115186

GTX115186 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115186) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP14 antibody used in Western Blot (WB). GTX115186

GTX115186 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: JurKat
7.5% SDS PAGE
GTX115186 diluted at 1:1000

Anti-USP14 antibody used in Western Blot (WB). GTX115186

GTX115186 WB Image

USP14 antibody detects USP14 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
USP14 antibody (GTX115186) dilution: 1:1000

REFERENCE

Application Reference

Raz V et al. J Cachexia Sarcopenia Muscle 2017; Proteasomal activity-based probes mark protein homeostasis in muscles.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

USP14 antibody Cat. No. GTX115186

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-USP14 antibody used in Western Blot (WB). GTX115186
Date : Anonymous submitted on 13-May-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : K562 cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 10Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169