GeneTex
United States (US)

USP14 antibody

KO/KD
GTX115187_40254_20180330_WB_KO_watremark_18121410_883.jpg
GTX115187_40254_20170518_WB_M_R_18121410_337.jpg
GTX115187_40254_20170719_IFA_R_18121410_159.jpg
GTX115187_40254_IHC_18121410_565.jpg
GTX115187_WB_18121410_570.jpg
GTX115187_40254_20170608_IHC-P_M_18121410_406.jpg

Cat No. GTX115187

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HeLa , A431 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Specific Peptidase 14,Tgt,Usp14

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115187_40254_20180330_WB_KO_watremark_18121410_883.jpg

GTX115187 WB Image

KO/KD

Wild-type (WT) and USP14 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX115187_40254_20170518_WB_M_R_18121410_337.jpg

GTX115187 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115187_40254_20170719_IFA_R_18121410_159.jpg

GTX115187 ICC/IF Image

USP14 antibody detects USP14 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP14 protein stained by USP14 antibody (GTX115187) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115187_40254_IHC_18121410_565.jpg

GTX115187 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal liver, using USP14(GTX115187) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115187_WB_18121410_570.jpg

GTX115187 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX115187 diluted at 1:1000

GTX115187_40254_20170608_IHC-P_M_18121410_406.jpg

GTX115187 IHC-Fr Image

USP14 antibody detects USP14 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: USP14 protein stained by USP14 antibody (GTX115187) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169