GeneTex
United States (US)

USP15 antibody [C3], C-term

GTX104644_43040_20171220_ICC_IF_19011506_833.jpg
GTX104644_43040_20171117_WB_19011506_983.jpg
GTX104644_IHC_19011506_229.jpg
GTX104644_39890_WB_M_19011506_181.jpg

Cat No. GTX104644

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

K562 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human USP15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Specific Peptidase 15,Unph-2,Unph4,Usp15

Background

Ubiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).[supplied by OMIM]

Database

DATA IMAGES
GTX104644_43040_20171220_ICC_IF_19011506_833.jpg

GTX104644 ICC/IF Image

USP15 antibody [C3], C-term detects USP15 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP15 stained by USP15 antibody [C3], C-term (GTX104644) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX104644_43040_20171117_WB_19011506_983.jpg

GTX104644 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with USP15 antibody [C3], C-term (GTX104644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104644_IHC_19011506_229.jpg

GTX104644 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using USP15(GTX104644) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104644_39890_WB_M_19011506_181.jpg

GTX104644 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX104644 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169