GeneTex
United States (US)

USP18 antibody

GTX103145_WB_19011506_720.jpg
GTX103145_39918_IHC_19011506_564.jpg
GTX103145_39918_IFA_19011506_784.jpg

Cat No. GTX103145

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

IMR32 , HCT116
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ISG43 antibody, UBP43 antibody, USP18 antibody, ubiquitin specific protease 18 antibody, ubl carboxyl-terminal hydrolase 18 antibody, ubl thioesterase 18 antibody, hUBP43 antibody, ubl thiolesterase 18 antibody, 43 kDa ISG15-specific protease antibody, ISG15-specific-processing protease antibody, ubiquitin specific peptidase 18 antibody

Background

USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.[supplied by OMIM]

Database

DATA IMAGES
GTX103145_WB_19011506_720.jpg

GTX103145 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32 10% SDS PAGE
GTX103145 diluted at 1:1000

GTX103145_39918_IHC_19011506_564.jpg

GTX103145 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using USP18(GTX103145) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103145_39918_IFA_19011506_784.jpg

GTX103145 ICC/IF Image

USP18 antibody detects USP18 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP18 protein stained by USP18 antibody (GTX103145) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169