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USP22 antibody [N3C3]

Anti-USP22 antibody [N3C3] used in Immunoprecipitation (IP). GTX120048
Anti-USP22 antibody [N3C3] used in Western Blot (WB). GTX120048

Cat. No. GTX120048

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all USP22 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724115

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 22 , USP3L

Background

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.

Database

Research Area

DATA IMAGES

Anti-USP22 antibody [N3C3] used in Immunoprecipitation (IP). GTX120048

GTX120048 IP Image

Immunoprecipitation of USP22 protein from A549 whole cell extracts using 5 μg of USP22 antibody [N3C3] (GTX120048).
Western blot analysis was performed using USP22 antibody [N3C3] (GTX120048) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-USP22 antibody [N3C3] used in Western Blot (WB). GTX120048

GTX120048 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX120048 diluted at 1:1000

REFERENCE

Application Reference

Wang Y et al. EMBO Rep 2019; 20 (7) : e47563 Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.
Wang Y et al. EMBO Rep 2019; (Epub) Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.
Application : WB
Reactivity : Human
Chen S et al. Nucleic Acids Res 2016; 45 (3) : 1144-1158. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169