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USP25 antibody

Anti-USP25 antibody used in Western Blot (WB). GTX17216
Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX17216
Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX17216

Cat. No. GTX17216

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all USP25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Mouse Brain Tissue Lysate(GTX27934)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25.The immunogen is located within amino acids 470 - 520 of USP25.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 25 , USP21

Cellular Localization

Cytoplasm,Nucleus

Background

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-USP25 antibody used in Western Blot (WB). GTX17216

GTX17216 WB Image

WB analysis of mouse brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX17216 USP25 antibody.
Working concentration : 1 μg/ml

Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX17216

GTX17216 IHC-P Image

IHC-P analysis of human brain tissue using GTX17216 USP25 antibody.
Working concentration : 20 μg/ml

Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX17216

GTX17216 IHC-P Image

IHC-P analysis of human brain tissue using GTX17216 USP25 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399