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USP3 antibody

Anti-USP3 antibody used in Western Blot (WB). GTX128238
Anti-USP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128238
Anti-USP3 antibody used in Western Blot (WB). GTX128238

Cat No. GTX128238

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , HeLa , human USP3-transfected 293T

Predict Reactivity

Mouse, Rat, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin specific peptidase 3 , SIH003 , UBP

Background

Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.

Database

Research Area

DATA IMAGES

Anti-USP3 antibody used in Western Blot (WB). GTX128238

GTX128238 WB Image

USP3 antibody detects USP3 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
USP3 antibody (GTX128238) dilution: 1:1000

Anti-USP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128238

GTX128238 ICC/IF Image

USP3 antibody detects USP3 protein at nucleus by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP3 protein stained by USP3 antibody (GTX128238) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-USP3 antibody used in Western Blot (WB). GTX128238

GTX128238 WB Image

USP3 antibody detects USP3 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human USP3-transfected 293T cells
7.5 % SDS-PAGE
USP3 antibody (GTX128238) dilution: 1:20000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169