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USP3 antibody

Orthogonal Overexpression
Anti-USP3 antibody used in Western Blot (WB). GTX128238
Anti-USP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128238
Anti-USP3 antibody used in Western Blot (WB). GTX128238
Anti-USP3 antibody used in Western Blot (WB). GTX128238

Cat. No. GTX128238

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all USP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , HeLa , human USP3-transfected 293T cells

Predict Reactivity

Mouse, Rat, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885735

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 3 , SIH003 , UBP

Background

Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.

Database

Research Area

DATA IMAGES

Anti-USP3 antibody used in Western Blot (WB). GTX128238

GTX128238 WB Image

USP3 antibody detects USP3 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
USP3 antibody (GTX128238) dilution: 1:1000

Anti-USP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128238

GTX128238 ICC/IF Image

USP3 antibody detects USP3 protein at nucleus by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP3 protein stained by USP3 antibody (GTX128238) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-USP3 antibody used in Western Blot (WB). GTX128238

GTX128238 WB Image

Overexpression

USP3 antibody detects USP3 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human USP3-transfected 293T cells
7.5 % SDS-PAGE
USP3 antibody (GTX128238) dilution: 1:20000

Anti-USP3 antibody used in Western Blot (WB). GTX128238

GTX128238 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2017.PMID: 28881649

REFERENCE

Application Reference

JK Karapurkar et al. J Exp Clin Cancer Res 2023; 42 (1) : 121 CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis
Application : ICC/IF
Reactivity : Human
Chandrasekaran AP et al. Mol Ther 2022; 30 (11) : 3414-3429 Dual role of deubiquitinating enzyme USP19 regulates mitotic progression and tumorigenesis by stabilizing survivin.
Byung-Ho Rhie et al. Int J Mol Sci 2021; 22 (11) Ubiquitin-Specific Protease 3 Deubiquitinates and Stabilizes Oct4 Protein in Human Embryonic Stem Cells.
Application : WB
Reactivity : Human
Das S et al. Cell Death Differ 2020; 27 (11) : 3004-3020 Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.
Application : ICC/IF
Cheng YC et al. Proc Natl Acad Sci U S A 2018; 115 (21) : 5546-5551 Deubiquitinating enzyme USP3 controls CHK1 chromatin association and activation.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169