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USP4 antibody

Anti-USP4 antibody used in Western Blot (WB). GTX103237
Anti-USP4 antibody used in Western Blot (WB). GTX103237
Anti-USP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103237
Anti-USP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103237

Cat No. GTX103237

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

Positive Control

U87-MG , MCF-7

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ubiquitin specific peptidase 4 , UNP , Unph

Cellular Localization

Cytoplasm

Database

DATA IMAGES
Anti-USP4 antibody used in Western Blot (WB). GTX103237

GTX103237 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX103237 diluted at 1:1000

Anti-USP4 antibody used in Western Blot (WB). GTX103237

GTX103237 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with USP4 antibody (GTX103237) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-USP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103237

GTX103237 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using USP4(GTX103237) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX103237

GTX103237 IHC-P Image

USP4 antibody detects USP4 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
USP4 antibody (GTX103237) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169