GeneTex
United States (US)

USP44 antibody

USP44-antibody-GTX87933-WB-1_18122102_695.jpg

Cat No. GTX87933

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

81 kDa. ( Note )

Specificity/Sensitivity

USP44 Antibody detects endogenous levels of total USP44 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human USP44.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Specific Peptidase 44,Usp44

Background

Deubiquitinase that plays a key role in the spindle checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint), thereby preventing premature activation of the APC/C.

Database

Research Area

DATA IMAGES
USP44-antibody-GTX87933-WB-1_18122102_695.jpg

GTX87933 WB Image

Western blot analysis of extracts from LOVO/NIH-3T3 cells, using USP44 Antibody. The lane on the right is treated with the synthesized peptide.

REFERENCE
Package List Price ($)
$ 339