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USP46 antibody

Anti-USP46 antibody used in IHC (Paraffin sections) (IHC-P). GTX117994
Anti-USP46 antibody used in Western Blot (WB). GTX117994
Anti-USP46 antibody used in Western Blot (WB). GTX117994

Cat. No. GTX117994

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all USP46 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HepG2 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP46. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166813

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 46

Background

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-USP46 antibody used in IHC (Paraffin sections) (IHC-P). GTX117994

GTX117994 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using USP46(GTX117994) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP46 antibody used in Western Blot (WB). GTX117994

GTX117994 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX117994 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP46 antibody used in Western Blot (WB). GTX117994

GTX117994 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX117994 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang Y et al. EMBO Rep 2019; 20 (7) : e47563 Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.
Wang Y et al. EMBO Rep 2019; (Epub) Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.
Application : WB
Reactivity : Human
Chen S et al. Nucleic Acids Res 2016; 45 (3) : 1144-1158. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169