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USP47 antibody [C3], C-term

Anti-USP47 antibody [C3], C-term used in Western Blot (WB). GTX116278
Anti-USP47 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116278
Anti-USP47 antibody [C3], C-term used in Western Blot (WB). GTX116278

Cat. No. GTX116278

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all USP47 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

157 kDa. ( Note )

Positive Control

Mouse brain , U87-MG

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP47. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624687

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 47 , TRFP

Background

Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues.

Database

Research Area

DATA IMAGES

Anti-USP47 antibody [C3], C-term used in Western Blot (WB). GTX116278

GTX116278 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX116278 diluted at 1:1000

Anti-USP47 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116278

GTX116278 IHC-P Image

USP47 antibody [C3], C-term detects USP47 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
USP47 antibody [C3], C-term (GTX116278) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP47 antibody [C3], C-term used in Western Blot (WB). GTX116278

GTX116278 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX116278 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169