GeneTex
United States (US)

USP47 antibody

GTX106253_39939_IFA_19011506_669.jpg
GTX106253_39939_WB_M_brain_19011506_989.jpg
GTX106253_WB_19011506_546.jpg

Cat No. GTX106253

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

157 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP47. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686C13257 antibody, FLJ20727 antibody, TRFP antibody, USP47 antibody, ubiquitin carboxyl-terminal hydrolase 47 antibody, ubiquitin-specific-processing protease 47 antibody, Trf (TATA binding protein-related factor)-proximal homolog antibody, ubiquitin specific protease 47 antibody, deubiquitinating enzyme 47 antibody, ubiquitin thiolesterase 47 antibody, ubiquitin thioesterase 47 antibody, ubiquitin specific peptidase 47 antibody

Background

Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues.
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Database

DATA IMAGES
GTX106253_39939_IFA_19011506_669.jpg

GTX106253 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using USP47(GTX106253) antibody at 1:200 dilution.

GTX106253_39939_WB_M_brain_19011506_989.jpg

GTX106253 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX106253 diluted at 1:1000

GTX106253_WB_19011506_546.jpg

GTX106253 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
5% SDS PAGE
USP47 antibody
GTX106253 diluted at 1:1000

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169