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USP5 antibody [2F5]

Anti-USP5 antibody [2F5] used in Western Blot (WB). GTX83431
Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431
Anti-USP5 antibody [2F5] used in Western Blot (WB). GTX83431
Anti-USP5 antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83431
Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431
Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431
Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431

Cat. No. GTX83431

Host

Mouse

Clonality

Monoclonal

Clone Name

2F5

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human USP5(NP_003472) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 5 , ISOT

Background

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition.

Database

Research Area

DATA IMAGES

Anti-USP5 antibody [2F5] used in Western Blot (WB). GTX83431

GTX83431 WB Image

WB analysis of HEK293T cells transfected with USP5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83431 USP5 antibody [2F5].
Loading : 5 ug per lane

Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431

GTX83431 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83431 USP5 antibody [2F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-USP5 antibody [2F5] used in Western Blot (WB). GTX83431

GTX83431 WB Image

WB analysis of various cell lines using GTX83431 USP5 antibody [2F5].
Loading : 35 ug per lane
Dilution : 1:200

Anti-USP5 antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83431

GTX83431 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with USP5 plasmid using GTX83431 USP5 antibody [2F5].

Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431

GTX83431 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX83431 USP5 antibody [2F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431

GTX83431 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX83431 USP5 antibody [2F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-USP5 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83431

GTX83431 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83431 USP5 antibody [2F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

There are currently no references for USP5 antibody [2F5] (GTX83431). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for USP5 antibody [2F5] (GTX83431). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519