GeneTex
United States (US)

USP5 antibody [C1C3]

GTX105407_40002_WB_M_brain_19011506_847.jpg
GTX105407_40002_IHC_19011506_954.jpg
GTX105407_WB_19011506_954.jpg
GTX105407_40002_20150410_IHC_M_19011506_128.jpg

Cat No. GTX105407

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ISOT antibody, USP5 antibody, isopeptidase T antibody, ubiquitin thiolesterase 5 antibody, deubiquitinating enzyme 5 antibody, ubiquitin isopeptidase T antibody, ubiquitin specific protease 5 (isopeptidase T) antibody, ubiquitin-specific-processing protease 5 antibody, ubiquitin-specific protease-5 (ubiquitin isopeptidase T) antibody, ubiquitin thioesterase 5 antibody, ubiquitin carboxyl-terminal hydrolase 5 antibody, ubiquitin specific peptidase 5 (isopeptidase T) antibody

Background

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM]

Database

DATA IMAGES
GTX105407_40002_WB_M_brain_19011506_847.jpg

GTX105407 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105407 diluted at 1:1000

GTX105407_40002_IHC_19011506_954.jpg

GTX105407 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using USP5(GTX105407) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105407_WB_19011506_954.jpg

GTX105407 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
7.5% SDS PAGE
GTX105407 diluted at 1:1000

GTX105407_40002_20150410_IHC_M_19011506_128.jpg

GTX105407 IHC-P Image

USP5 antibody [C1C3] detects USP5 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
USP5 antibody [C1C3] (GTX105407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169