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USP5 antibody [C1C3]

Anti-USP5 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105407
Anti-USP5 antibody [C1C3] used in Western Blot (WB). GTX105407
Anti-USP5 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105407
Anti-USP5 antibody [C1C3] used in Western Blot (WB). GTX105407

Cat. No. GTX105407

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all USP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

Mouse brain , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952531

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 5 , ISOT

Background

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-USP5 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105407

GTX105407 IHC-P Image

USP5 antibody [C1C3] detects USP5 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
USP5 antibody [C1C3] (GTX105407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP5 antibody [C1C3] used in Western Blot (WB). GTX105407

GTX105407 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
7.5% SDS PAGE
GTX105407 diluted at 1:1000

Anti-USP5 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105407

GTX105407 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using USP5(GTX105407) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP5 antibody [C1C3] used in Western Blot (WB). GTX105407

GTX105407 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105407 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169