GeneTex

  • Country / Location Selection

United States (US)

USP7 antibody [GT481]

Anti-USP7 antibody [GT481] used in Western Blot (WB). GTX631108
Anti-USP7 antibody [GT481] used in Immunoprecipitation (IP). GTX631108
Anti-USP7 antibody [GT481] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631108

Cat. No. GTX631108

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT481

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all USP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888241

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 7 , HAUSP , TEF1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-USP7 antibody [GT481] used in Western Blot (WB). GTX631108

GTX631108 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP7 antibody [GT481] (GTX631108) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-USP7 antibody [GT481] used in Immunoprecipitation (IP). GTX631108

GTX631108 IP Image

Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody [GT481] (GTX631108).
Western blot analysis was performed using USP7 antibody [GT481] (GTX631108).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-USP7 antibody [GT481] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631108

GTX631108 ICC/IF Image

USP7 antibody [GT481] detects USP7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 stained by USP7 antibody [GT481] (GTX631108) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Scale bar= 10μm.

REFERENCE

Application Reference

Wang Y et al. EMBO Rep 2019; 20 (7) : e47563 Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.
Wang Y et al. EMBO Rep 2019; (Epub) Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.
Application : WB, ChIP assay
Reactivity : Human
Xiang Q et al. J Virol 2018; (Epub) human herpesvirus 8 * regulatory factors 1 and 3 mediate replication and latency activities via interactions with usp7 deubiquitinase.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for USP7 antibody [GT481] (GTX631108). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169