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USP7 antibody [GT6512]

Anti-USP7 antibody [GT6512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631109
Anti-USP7 antibody [GT6512] used in Immunoprecipitation (IP). GTX631109
Anti-USP7 antibody [GT6512] used in Western Blot (WB). GTX631109

Cat. No. GTX631109

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT6512

Isotype

 IgG2b

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all USP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888242

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 7 , HAUSP , TEF1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-USP7 antibody [GT6512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631109

GTX631109 ICC/IF Image

USP7 antibody [GT6512] detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 protein stained by USP7 antibody [GT6512] (GTX631109) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-USP7 antibody [GT6512] used in Immunoprecipitation (IP). GTX631109

GTX631109 IP Image

Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody [GT6512] (GTX631109).
Western blot analysis was performed using USP7 antibody [GT6512] (GTX631109).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-USP7 antibody [GT6512] used in Western Blot (WB). GTX631109

GTX631109 WB Image

USP7 antibody [GT6512] detects USP7 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
5 % SDS-PAGE
USP7 antibody [GT6512] (GTX631109) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations