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USP7 antibody

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Immunoprecipitation (IP). GTX125894

Cat No. GTX125894

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all USP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , USP7 transfected 293T lysate , NIH-3T3 , JC , PC-12

Predict Reactivity

Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin specific peptidase 7 , HAUSP , TEF1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
5% SDS PAGE
GTX125894 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894

GTX125894 ICC/IF Image

USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 protein stained by USP7 antibody (GTX125894) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A:  Non-transfected 293T lysates 
B:  USP7 transfected 293T lysates
7.5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Immunoprecipitation (IP). GTX125894

GTX125894 IP Image

Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody (GTX125894).
Western blot analysis was performed using USP7 antibody (GTX125894).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Verwey N et al. Nucleic Acid Ther 2019; (Epub) Antisense-Mediated Skipping of Dysferlin Exons in Control and Dysferlinopathy Patient-Derived Cells.
Application : WB
Ji L et al. Nat Commun 2019; 10 (1) : 4184 USP7 inhibits Wnt/β-catenin signaling through promoting stabilization of Axin.
Application : IP
Reactivity : Human
Vishnoi M et al. Cancer Res 2018; (Epub) Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.
Application : ICC/IF
Reactivity : Mouse
Chen S et al. Nucleic Acids Res 2016; 45 (3) : 1144-1158. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169