GeneTex
United States (US)

USP7 antibody

GTX125894_40996_IHC_M_3_18121410_430.jpg
GTX125894_40996_WB_M_18121410_929.jpg
GTX125894_40996_IHC_M_2_18121410_859.jpg
GTX125894_40996_WB_18121410_879.jpg
GTX125894_40996_IHC_M_18121410_554.jpg
GTX125894_40996_WB_R_18121410_693.jpg
GTX125894_41010_IFA_18121410_262.jpg
GTX125894_40996_WB_B_18121410_533.jpg
GTX125894_40996_20150717_IP_18121410_956.jpg

Cat No. GTX125894

Reference 3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
See all USP7 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , USP7 transfected 293T lysate , NIH-3T3 , JC , PC-12

Predict Reactivity

Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HAUSP antibody, TEF1 antibody, USP7 antibody, ubiquitin specific protease 7 (herpes virus-associated) antibody, ubiquitin-specific-processing protease 7 antibody, Herpes virus-associated ubiquitin-specific protease antibody, ubiquitin carboxyl-terminal hydrolase 7 antibody, herpesvirus-associated ubiquitin-specific protease antibody, deubiquitinating enzyme 7 antibody, ubiquitin thioesterase 7 antibody, ubiquitin specific peptidase 7 (herpes virus-associated) antibody

Cellular Localization

Nucleus

Database

DATA IMAGES
GTX125894_40996_IHC_M_3_18121410_430.jpg

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX125894_40996_WB_M_18121410_929.jpg

GTX125894 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX125894_40996_IHC_M_2_18121410_859.jpg

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX125894_40996_WB_18121410_879.jpg

GTX125894 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
5% SDS PAGE
GTX125894 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX125894_40996_IHC_M_18121410_554.jpg

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX125894_40996_WB_R_18121410_693.jpg

GTX125894 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX125894_41010_IFA_18121410_262.jpg

GTX125894 ICC/IF Image

USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 protein stained by USP7 antibody (GTX125894) diluted at 1:500.
Blue: Hoechst 33343 staining.
GTX125894_40996_WB_B_18121410_533.jpg

GTX125894 WB Image

Sample (30 µg of whole cell lysate)
A:  Non-transfected 293T lysates 
B:  USP7 transfected 293T lysates
7.5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX125894_40996_20150717_IP_18121410_956.jpg

GTX125894 IP Image

Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody (GTX125894).
Western blot analysis was performed using USP7 antibody (GTX125894).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Reference

Application Reference

Vishnoi M et al. Cancer Res 2018; (Epub ahead of print) Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.
Application : ICC/IF
Reactivity : Mouse
Chen S et al. Nucleic Acids Res 2017; 45 (3):1144-1158 Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy.
Chen S et al. Nucleic Acids Res 2016; 45 (3):1144-1158. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy.
Application : WB
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169