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USP7 antibody

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Immunoprecipitation (IP). GTX125894

Cat No. GTX125894

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , USP7 transfected 293T lysate , NIH-3T3 , JC , PC-12

Predict Reactivity

Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885611

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin specific peptidase 7 , HAUSP , TEF1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
5% SDS PAGE
GTX125894 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894

GTX125894 ICC/IF Image

USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 protein stained by USP7 antibody (GTX125894) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A:  Non-transfected 293T lysates 
B:  USP7 transfected 293T lysates
7.5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Immunoprecipitation (IP). GTX125894

GTX125894 IP Image

Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody (GTX125894).
Western blot analysis was performed using USP7 antibody (GTX125894).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

REVIEW

USP7 antibody Cat No. GTX125894

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31519875
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf