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USP7 antibody

Orthogonal
Anti-USP7 antibody used in Western Blot (WB). GTX24080
Anti-USP7 antibody used in Western Blot (WB). GTX24080
Anti-USP7 antibody used in ELISA (ELISA). GTX24080

Cat. No. GTX24080

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all USP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:15000-1:60000
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids near the amino terminus of human HAUSP protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 7 , HAUSP , TEF1

Cellular Localization

Nucleus,Cytoplasm

Background

HAUSP is a novel p53 interacting protein identified by mass spectrometry of affinity-purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53 even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Database

Research Area

DATA IMAGES

Anti-USP7 antibody used in Western Blot (WB). GTX24080

GTX24080 WB Image

Western blot using GeneTex affinity purified anti-HAUSP antibody (GTX24080) shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.

Anti-USP7 antibody used in Western Blot (WB). GTX24080

GTX24080 WB Image

Orthogonal

WB analysis of various samples using GTX24080 USP7 antibody prior incubated with (Lane 6-10) or without (Lane 1-5) blocking peptide.Lane 1 and 6 : HeLa nuclear extracts
Lane 2 and 7 : HeLa whole cell lysates
Lane 3 and 8 : A431 whole cell lysates
Lane 4 and 9 : MCF-7 whole cell lysates
Lane 5 and 10 : NIH-3T3 whole cell lysates
Dilution : 1:500

Anti-USP7 antibody used in ELISA (ELISA). GTX24080

GTX24080 ELISA Image

ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX24080 USP7 antibody.
Coating : 0.1 μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399