ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX24080 USP7 antibody.
Coating : 0.1 μg

WB analysis of various samples using GTX24080 USP7 antibody prior incubated with (Lane 6-10) or without (Lane 1-5) blocking peptide.Lane 1 and 6 : HeLa nuclear extracts
Lane 2 and 7 : HeLa whole cell lysates
Lane 3 and 8 : A431 whole cell lysates
Lane 4 and 9 : MCF-7 whole cell lysates
Lane 5 and 10 : NIH-3T3 whole cell lysates
Dilution : 1:500

Western blot using GeneTex affinity purified anti-HAUSP antibody (GTX24080) shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.