GeneTex
United States (US)

USP8 antibody

GTX103747_40688_IP_19011506_929.jpg
GTX103747_40688_WB_19011506_432.jpg
GTX103747_40688_IFA_19011506_589.jpg
GTX103747_40688_IHC_19011506_629.jpg

Cat No. GTX103747

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

H1299 , HCT116

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USP8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Specific Peptidase 8,Humorf8,Pita4,Spg59,Ubpy,Usp8

Database

Research Area

DATA IMAGES
GTX103747_40688_IP_19011506_929.jpg

GTX103747 IP Image

Immunoprecipitation of USP8 protein from HCT-116 whole cell extracts using 5 μg of USP8 antibody (GTX103747).
Western blot analysis was performed using USP8 antibody (GTX103747).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103747_40688_WB_19011506_432.jpg

GTX103747 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: HCT116
5% SDS PAGE
GTX103747 diluted at 1:5000

GTX103747_40688_IFA_19011506_589.jpg

GTX103747 ICC/IF Image

USP8 antibody detects USP8 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP8 protein stained by USP8 antibody (GTX103747) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103747_40688_IHC_19011506_629.jpg

GTX103747 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using USP8(GTX103747) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169