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USP9x antibody [JG35-11]

Recombinant antibody
Anti-USP9x antibody [JG35-11] used in Western Blot (WB). GTX01008
Anti-USP9x antibody [JG35-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01008
Anti-USP9x antibody [JG35-11] used in Flow cytometry (FACS). GTX01008
Anti-USP9x antibody [JG35-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01008
Anti-USP9x antibody [JG35-11] used in IHC (Paraffin sections) (IHC-P). GTX01008
Anti-USP9x antibody [JG35-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01008
Anti-USP9x antibody [JG35-11] used in IHC (Paraffin sections) (IHC-P). GTX01008

Cat No. GTX01008

Host

Rabbit

Clonality

Monoclonal

Clone Name

JG35-11

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

292 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cytoplasm,Cell projection,Growth cone

Background

This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-USP9x antibody [JG35-11] used in Western Blot (WB). GTX01008

GTX01008 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP9x antibody [JG35-11] (GTX01008) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP9x antibody [JG35-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01008

GTX01008 ICC/IF Image

ICC/IF analysis of PFA-fixed 293T cells using GTX01008 USP9x antibody [JG35-11].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-USP9x antibody [JG35-11] used in Flow cytometry (FACS). GTX01008

GTX01008 FACS Image

FACS analysis of 293T cells using GTX01008 USP9x antibody [JG35-11].
Purple : primary antibody
Yellow : unlabelled control
Dilution : 1:100

Anti-USP9x antibody [JG35-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01008

GTX01008 ICC/IF Image

ICC/IF analysis of PFA-fixed SiHa cells using GTX01008 USP9x antibody [JG35-11].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-USP9x antibody [JG35-11] used in IHC (Paraffin sections) (IHC-P). GTX01008

GTX01008 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01008 USP9x antibody [JG35-11].
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins.

Anti-USP9x antibody [JG35-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01008

GTX01008 ICC/IF Image

ICC/IF analysis of PFA-fixed F9 cells using GTX01008 USP9x antibody [JG35-11].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-USP9x antibody [JG35-11] used in IHC (Paraffin sections) (IHC-P). GTX01008

GTX01008 IHC-P Image

IHC-P analysis of mouse fallopian tube tissue section using GTX01008 USP9x antibody [JG35-11].
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins.

REFERENCE

There are currently no references for USP9x antibody [JG35-11] (GTX01008). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for USP9x antibody [JG35-11] (GTX01008). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369