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UVSSA antibody [GT816]

Anti-UVSSA antibody [GT816] used in Western Blot (WB). GTX629742
Anti-UVSSA antibody [GT816] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629742
Anti-UVSSA antibody [GT816] used in Western Blot (WB). GTX629742

Cat. No. GTX629742

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT816

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UVSSA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

HeLa , A375

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human UVSSA. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888144

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UV stimulated scaffold protein A , KIAA1530 , UVSS3

Background

The protein encoded by this gene appears to be involved in ubiquitination and dephosphorylation of RNA polymerase II subunits that stall after UV irradiation. The encoded protein interacts with several members of the nucleotide excision repair complex, and is thought to be involved in the transcription-coupled nucleotide excision repair (TC-NER) pathway to help remove lesions in the DNA that block transcription. Defects in this gene can cause UV-sensitive syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-UVSSA antibody [GT816] used in Western Blot (WB). GTX629742

GTX629742 WB Image

UVSSA antibody [GT816] detects UVSSA protein by Western blot analysis.
A. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
UVSSA antibody [GT816] (GTX629742) dilution: 1:500

Anti-UVSSA antibody [GT816] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629742

GTX629742 ICC/IF Image

UVSSA antibody [GT816] detects UVSSA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UVSSA stained by UVSSA antibody [GT816] (GTX629742) diluted at 1:500.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-UVSSA antibody [GT816] used in Western Blot (WB). GTX629742

GTX629742 WB Image

UVSSA antibody [GT816] detects UVSSA protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
UVSSA antibody [GT816] (GTX629742) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations