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Ube2J1 antibody

Anti-Ube2J1 antibody used in  (). GTX48665
Anti-Ube2J1 antibody used in Western Blot (WB). GTX48665

Cat No. GTX48665

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Human
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:2000
IP 1 - 2 μg
ELISA 1:1000 - 1:5000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 J1 , CGI-76 , HSPC153 , HSPC205 , HSU93243 , NCUBE-1 , NCUBE1 , UBC6 , UBC6E , Ubc6p

Cellular Localization

Endoplasmic reticulum membrane

Background

Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.

Database

DATA IMAGES

Anti-Ube2J1 antibody used in  (). GTX48665

GTX48665 Image

Anti-Ube2J1 antibody used in Western Blot (WB). GTX48665

GTX48665 WB Image

Western blot using GeneTex's affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over-expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 289