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Ubiquilin 4 antibody

KO/KD
Anti-Ubiquilin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85267
Anti-Ubiquilin 4 antibody used in Western Blot (WB). GTX85267

Cat. No. GTX85267

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Ubiquilin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

3T3 (NIH) Cell Lysate(GTX27179)

Product Note

Knockdown/Knockout validation was supported by references (PMID:36951542)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CIP75 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75.The immunogen is located within the first 50 amino acids of CIP75.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquilin 4 , A1U , A1Up , C1orf6 , CIP75 , UBIN

Cellular Localization

Nucleus,Cytoplasm,Endoplasmic reticulum

Background

The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.

Database

Research Area

DATA IMAGES

Anti-Ubiquilin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX85267

GTX85267 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX85267 Ubiquilin 4 antibody.
Working concentration : 5 μg/ml

Anti-Ubiquilin 4 antibody used in Western Blot (WB). GTX85267

GTX85267 WB Image

WB analysis of 3T3 cell lysate using GTX85267 Ubiquilin 4 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

Application Reference

Black HH et al. Elife 2023; 12 UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399