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Ubiquitin antibody [GT7811]

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148
Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

Cat No. GTX630148

Host

Mouse

Clonality

Monoclonal

Clone Name

GT7811

Isotype

IgG2b

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Yeast, Drosophila, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Ubiquitin.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888189

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:5000.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Ubiquitin antibody detects Ubiquitin protein by western blot analysis. Non-transfected (-) and -transfected (+, including Myc-tag) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody (GTX630148) diluted by 1:10000.

Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148

GTX630148 IHC-P Image

Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148

GTX630148 IHC-P Image

Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:500 and competitor's antibody (sc-53509) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169