*Optimal dilutions/concentrations should be determined by the researcher.
Application
Dilution
WB
1:5000-1:20000
IHC-P
1:100-1:1000
Not tested in other applications.
Predict Reactivity
Yeast, Drosophila, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
The immunogen used to generate this antibody corresponds to Ubiquitin.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888189
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:5000.
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GTX630148 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX630148 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX630148 WB Image
Ubiquitin antibody detects Ubiquitin protein by western blot analysis. Non-transfected (-) and -transfected (+, including Myc-tag) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody (GTX630148) diluted by 1:10000.
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GTX630148 IHC-P Image
Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630148 IHC-P Image
Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human endometrial carcinoma.Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630148 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:500 and competitor's antibody (sc-53509) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX630148 WB Image
293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.