GeneTex
United States (US)

Ubiquitin antibody

Orthogonal
GTX30439_553_IHC-P_18121410_254.jpg
GTX30439_208_ICC-IF_18121410_388.jpg
GTX30439_1400_WB_18121410_506.jpg

Cat No. GTX30439

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, ELISA
Reactivity Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000 - 1:10000
ICC/IF 1:500 - 1:1000
IHC-P 1:500 - 1:1000
IHC-Fr 1:500 - 1:1000
ELISA 1:100 - 1:2000
Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes ubiquitinated inclusion bodies and both the mono- and polyubiquitin forms. This recognizes aggresomes.
PROPERTIES

Form

Liquid

Buffer

Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Immunogen

Gluteraldehyde cross-linked Ubiquitin.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX30439_553_IHC-P_18121410_254.jpg

GTX30439 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX30439 Ubiquitin antibody.

GTX30439_208_ICC-IF_18121410_388.jpg

GTX30439 ICC/IF Image

ICC/IF analysis of mouse L-cells treated with lactacysteine using GTX30439 Ubiquitin antibody.

GTX30439_1400_WB_18121410_506.jpg

GTX30439 WB Image

Orthogonal

WB analysis of HEK293 cell lysate treated with proteasome inhibitor lactacystin (Lc) at 10 um for 16 h using GTX30439 Ubiquitin antibody.
Green : ubiquitin
Red : beta Tubulin
Dilution : 1:5000

Package List Price ($)
$ 339