Ulp1 antibody

WB analysis of recombinant yeast Ulp1 protein using GTX48820 Ulp1 antibody.
Dilution : 1:1000

WB analysis of recombinant yeast Ulp1 protein using GTX48820 Ulp1 antibody.
Lane 1 : 50 ng
Lane 2 : 25 ng
Lane 3 : 12.5 ng
Lane 4 : 6.25 ng
Lane 5 : 3.13 ng
Dilution : 1:1000

Western blot using GeneTex's Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 μg of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TBST, the blot was probed overnight at 4ºC with a 1:1,000 dilution of anti-yULP1 antibody detected as above. This antibody is specific for yeast ULP1 and does not react with ULP1 from related sources including human SENP.

Western blot using GeneTex's Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TBST, and probed overnight at 4ºC with a 1:1000 dilution of anti-yULP-1 antibody in 5% non-fat dry milk in TBST. Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.

Western Blot of Rabbit Anti-ULP1 Antibody. (in duplicate) Lane 1: 50ng ULP1CD. Lane 2: 25ng ULP1CD. Lane 3: 12.5ng ULP1CD. Lane 4: 6.25ng ULP1CD. Lane 5: 3.13ng ULP1CD. Primary Antibody: Anti-ULP1 at 1:1,000 dilution O/N at 4ºC. Secondary Antibody: Goat Anti-Rabbit HRP 1:5,000 RT for 30 min.