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Urokinase antibody

Orthogonal
Anti-Urokinase antibody used in IHC (Paraffin sections) (IHC-P). GTX100465
Anti-Urokinase antibody used in Western Blot (WB). GTX100465
Anti-Urokinase antibody used in Western Blot (WB). GTX100465
Anti-Urokinase antibody used in IHC (Paraffin sections) (IHC-P). GTX100465

Cat. No. GTX100465

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Urokinase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

H1299 , HCT116 , PC3 conditioned medium

Predict Reactivity

Rabbit, Sheep, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Urokinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624144

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

plasminogen activator, urokinase , ATF , BDPLT5 , QPD , UPA , URK , u-PA

Cellular Localization

Secreted

Background

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Urokinase antibody used in IHC (Paraffin sections) (IHC-P). GTX100465

GTX100465 IHC-P Image

Urokinase antibody detects Urokinase protein at cytoplasm and extracellular space in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
Urokinase antibody (GTX100465) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Urokinase antibody used in Western Blot (WB). GTX100465

GTX100465 WB Image

Orthogonal

PC-3 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Urokinase antibody (GTX100465) diluted at 1:500.

Anti-Urokinase antibody used in Western Blot (WB). GTX100465

GTX100465 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Urokinase antibody (GTX100465) diluted at 1:500.

Anti-Urokinase antibody used in IHC (Paraffin sections) (IHC-P). GTX100465

GTX100465 IHC-P Image

Urokinase antibody detects PLAU protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Urokinase antibody (GTX100465) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169