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Usher antibody (C-terminal)

Anti-Usher antibody (C-terminal) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47843

Cat No. GTX47843

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IP, ELISA

Reactivity

Human, Mouse
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:250
IP 1:200
ELISA 1:4000
Not tested in other applications.

Calculated MW

576 kDa. ( Note )

Specificity/Sensitivity

This antibody will detect both Usherin isoform A and Usherin isoform B.

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine pH 7.4-7.9, 30% glycerol, BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide taken within amino acid region 1495-1545 on human Usherin isoform A protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

usherin , RP39 , US2 , USH2 , dJ1111A8.1

Background

This gene encodes a protein that contains laminin EGF motifs, a pentaxin domain, and many fibronectin type III motifs. The protein is found in the basement membrane, and may be important in development and homeostasis of the inner ear and retina. Mutations within this gene have been associated with Usher syndrome type IIa and retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-Usher antibody (C-terminal) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47843

GTX47843 ICC/IF Image

ICC/IF analysis of 2% PFA-fixed Oc-K1 cells using GTX47843 Usher antibody (C-terminal). Usher co-localizes with acetylated-tubulin at the basal body.
Cyan : Primary antibody
Purple : Acetyl-tubulin
Blue : Merged of Cyan and Purple
Yellow : DAPI
Dilution : 1:200 (overnight staining)

REFERENCE

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REVIEW

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Package List Price ($)
$ 429