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V-ATPase D antibody

Anti-V-ATPase D antibody used in Western Blot (WB). GTX119155
Anti-V-ATPase D antibody used in IHC (Paraffin sections) (IHC-P). GTX119155
Anti-V-ATPase D antibody used in Western Blot (WB). GTX119155

Cat. No. GTX119155

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATP6V1D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain , U87-MG

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human V-ATPase D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618185

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting V1 subunit D , ATP6M , VATD , VMA8

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-V-ATPase D antibody used in Western Blot (WB). GTX119155

GTX119155 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX119155 diluted at 1:1000

Anti-V-ATPase D antibody used in IHC (Paraffin sections) (IHC-P). GTX119155

GTX119155 IHC-P Image

V-ATPase D antibody detects ATP6V1D protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. V-ATPase D antibody (GTX119155) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-V-ATPase D antibody used in Western Blot (WB). GTX119155

GTX119155 WB Image

V-ATPase D antibody detects ATP6V1D protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
12 % SDS-PAGE
V-ATPase D antibody (GTX119155) dilution: 1:1000

REFERENCE

Application Reference

Domingo-Gonzalez R et al. JCI Insight 2017; 2 (19) Interleukin-17 limits hypoxia-inducible factor 1α and development of hypoxic granulomas during tuberculosis.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169