GeneTex
United States (US)

V-ATPase F antibody

GTX101768_40583_WB_19011506_748.jpg

Cat No. GTX101768

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human V-ATPase F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ATP6S14 antibody, MGC117321 antibody, MGC126037 antibody, MGC126038 antibody, VATF antibody, Vma7 antibody, ATP6V1F antibody, vacuolar ATP synthase subunit F antibody, H(+)-transporting two-sector ATPase, 14kD subunit antibody, ATPase, vacuolar, 14 kD antibody, V-ATPase subunit F antibody, V-ATPase F subunit antibody, vacuolar proton pump subunit F antibody, vacuolar proton pump F subunit antibody, V-type proton ATPase subunit F antibody, adenosinetriphosphatase 14k chain antibody, V-ATPase 14 kDa subunit antibody, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F antibody

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]
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Database

DATA IMAGES
GTX101768_40583_WB_19011506_748.jpg

GTX101768 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
15% SDS PAGE
GTX101768 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169