APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human V-ATPase F. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240498
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ATPase H+ transporting V1 subunit F , ATP6S14 , VATF , Vma7
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX101768 WB Image
Sample (30 ug of whole cell lysate)
A: U87-MG
15% SDS PAGE
GTX101768 diluted at 1:500
|
REFERENCE
There are currently no references for V-ATPase F antibody (GTX101768). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for V-ATPase F antibody (GTX101768). Be the first to share your experience with this product.
![Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624](/upload/website/prouct_img/small/GTX108624/GTX108624_43754_20191108_WB_KO_watermark_20092220_924.jpg "Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624")
