Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human V-ATPase F. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
ATP6S14 antibody, MGC117321 antibody, MGC126037 antibody, MGC126038 antibody, VATF antibody, Vma7 antibody, ATP6V1F antibody, vacuolar ATP synthase subunit F antibody, H(+)-transporting two-sector ATPase, 14kD subunit antibody, ATPase, vacuolar, 14 kD antibody, V-ATPase subunit F antibody, V-ATPase F subunit antibody, vacuolar proton pump subunit F antibody, vacuolar proton pump F subunit antibody, V-type proton ATPase subunit F antibody, adenosinetriphosphatase 14k chain antibody, V-ATPase 14 kDa subunit antibody, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F antibody
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]
Database
