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V5 tag antibody (Biotin)

Anti-V5 tag antibody (Biotin) used in ELISA (ELISA). GTX77436

Cat. No. GTX77436

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all V5 tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 0.01 ug/ml
ELISA 0.1 - 0.005 ug/ml
IHC 1.0 - 5.0 ug/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, stabilizer

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Rabbits were immunized with the V5 peptide conjugated with KLH. After multiple immunizations in Freunds adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase then conjugated with biotin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Biotin

RRID

AB_379983

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-V5 tag antibody (Biotin) used in ELISA (ELISA). GTX77436

GTX77436 ELISA Image

ELISA analysis of immunogen using GTX77436 V5 tag antibody (Biotin).

REFERENCE

Application Reference

Choi Y et al. J Virol 2020; 94 (6) Severe Fever with Thrombocytopenia Syndrome Virus NSs Interacts with TRIM21 To Activate the p62-Keap1-Nrf2 Pathway.
Choi Y et al. Nat Microbiol 2019; 4 (3) : 429-437 Severe fever with thrombocytopenia syndrome phlebovirus non-structural protein activates TPL2 signalling pathway for viral immunopathogenesis.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399