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V5 tag antibody [E10 / V4RR]

Anti-V5 tag antibody [E10 / V4RR] used in Western Blot (WB). GTX82563

Cat No. GTX82563

Host

Mouse

Clonality

Monoclonal

Clone Name

E10 / V4RR

Isotype

IgG1

Application

WB, ICC/IF, IP, ELISA, IHC
Package
100 μg ($339)

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes V5-tagged fusion proteins

PROPERTIES

Form

Liquid

Buffer

10mM PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Purification

Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

V5 epitope tag , GKPIPNPLLGLDST tag , V5 tag , GKPIPNPLLGLDST epitope tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Research Area

DATA IMAGES

Anti-V5 tag antibody [E10 / V4RR] used in Western Blot (WB). GTX82563

GTX82563 WB Image

1:000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)

REFERENCE

There are currently no references for V5 tag antibody [E10 / V4RR] (GTX82563). Be the first to share your publications with this product.

REVIEW

V5 tag antibody [E10 / V4RR] Cat No. GTX82563

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 04-Jun-2018
Score :
Application Tested : ICC/IF
Sample : N2a Cells
Fixation : 4% PFA, 4°C, 0.17Hr
Permeabilization /Antigen retrieval : 0.3% PBST, 25°C, 15 minHr
Primary Antibody : 1:100, 25°C, 1Hr
SDS
PBS.pdf
Package List Price ($)
$ 339