V5 tag antibody [E10 / V4RR]

Name: V5 tag antibody [E10 / V4RR]
Brand: GeneTex
SKU: GTX82563
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-V5 tag antibody [E10 / V4RR] used in Western Blot (WB). GTX82563

Cat. No. GTX82563

Host	Mouse
Clonality	Monoclonal
Clone Name	E10 / V4RR
Isotype	IgG1
Application	WB, ICC/IF, IP, ELISA, IHC
Package	100 μg ($399)

See all V5 tag products

Datasheet
Document

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes V5-tagged fusion proteins

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Purification

Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

V5 epitope tag , GKPIPNPLLGLDST tag , V5 tag , GKPIPNPLLGLDST epitope tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Research Area

DATA IMAGES

GTX82563 WB Image

1:000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)

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REVIEW