V5 tag antibody [GT1078]

Cat. No. GTX635260
1
Cat. No.  GTX635260
Anti-V5 tag antibody [GT1078] used in Immunoprecipitation (IP). GTX635260
GTX635260 IP Image

Immunoprecipitation of V5 tag protein from transfected 293T using 10 μg of V5 tag antibody [GT1078] (GTX635260).
Western blot analysis was performed using V5 tag antibody (GTX117997).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

1 / 3
Anti-V5 tag antibody [GT1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635260
GTX635260 ICC/IF Image

V5 tag antibody [GT1078] detects V5 tag protein by immunofluorescent analysis.
Sample: V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: V5 tag stained by V5 tag antibody [GT1078] (GTX635260) diluted at 1:2000.
Blue: Hoechst 33342 staining.

2 / 3
Anti-V5 tag antibody [GT1078] used in Western Blot (WB). GTX635260
GTX635260 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 Tag antibody [GT1078] (GTX635260) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

3 / 3
Anti-V5 tag antibody [GT1078] used in Immunoprecipitation (IP). GTX635260
Anti-V5 tag antibody [GT1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635260
Anti-V5 tag antibody [GT1078] used in Western Blot (WB). GTX635260
This V5 tag antibody [GT1078] (GTX635260) is a mouse monoclonal antibody validated for detection of V5 tag by WB, ICC/IF, and IP. Reactivity is species-independent.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT1078
  • Isotype
    IgG2a
  • Applications
    WB ICC/IF IP
  • Reactivity
    Species independent
See all V5 tag products

Ask