GeneTex
  • Country / Location Selection

United States (US)

V5 tag antibody [GT1078]

Anti-V5 tag antibody [GT1078] used in Western Blot (WB). GTX635260
Anti-V5 tag antibody [GT1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635260

Cat No. GTX635260

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1078

Isotype

IgG2a

Application

WB, ICC/IF
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

V5-tag tagged protein-transfected 293T

PROPERTIES

Form

Liquid

Buffer

20% Glycerol in PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to V5 tag

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888514

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-V5 tag antibody [GT1078] used in Western Blot (WB). GTX635260

GTX635260 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 Tag antibody [GT1078] (GTX635260) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-V5 tag antibody [GT1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635260

GTX635260 ICC/IF Image

V5 tag antibody [GT1078] detects V5 tag protein by immunofluorescent analysis.
Sample: V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: V5 tag stained by V5 tag antibody [GT1078] (GTX635260) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for V5 tag antibody [GT1078] (GTX635260). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for V5 tag antibody [GT1078] (GTX635260). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 329
$ 169