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V5 tag antibody [SV5-Pk1]

Anti-V5 tag antibody [SV5-Pk1] used in ELISA (ELISA). GTX42525
Anti-V5 tag antibody [SV5-Pk1] used in Western Blot (WB). GTX42525
Anti-V5 tag antibody [SV5-Pk1] used in  (). GTX42525

Cat No. GTX42525

Host

Mouse

Clonality

Monoclonal

Clone Name

SV5-Pk1

Isotype

IgG2a

Application

WB, ICC/IF, IHC-Fr, FACS, IP, ELISA, EMSA, RIA
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/1,000-1/5,000
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA 1/1,000-1/5,000
EMSA Assay dependent
RIA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Paramyxovirus Simian-Virus 5 (SV5)

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_510174

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

V/P Antibody

Research Area

DATA IMAGES

Anti-V5 tag antibody [SV5-Pk1] used in ELISA (ELISA). GTX42525

GTX42525 ELISA Image

ELISA analysis of V5 tagged protein using GTX42525 V5 tag antibody [SV5-Pk1].

Anti-V5 tag antibody [SV5-Pk1] used in Western Blot (WB). GTX42525

GTX42525 WB Image

WB analysis of V5 tagged protein using GTX42525 V5 tag antibody [SV5-Pk1].

Anti-V5 tag antibody [SV5-Pk1] used in  (). GTX42525

GTX42525 Image

REFERENCE

REVIEW

V5 tag antibody [SV5-Pk1] Cat No. GTX42525

Rating ( Average 4 based on 1 users reviews)
Application
Immunoprecipitation(IP) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 09-Oct-2012
Score :
Application Tested : IP
Sample : V5 fused target gene-transfected MDA-MB 468 cells
Amount used : 30 μg/well for input and 1000μg for IP(2μg/ul)
Blocking : 5% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A. 30 ug whole cell lysate/extract of transfected MDA-MB 468 cells, B. Control with 2 ug of pre-immune mouse IgG, C. Immunoprecipitation of V5 tagged recombinant protein by 2 ug of V5 tag antibody(GTX42525)
Figure Note : 2μg for IP
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339