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V5 tag antibody [SV5-Pk1]

Anti-V5 tag antibody [SV5-Pk1] used in Western Blot (WB). GTX42525
Anti-V5 tag antibody [SV5-Pk1] used in ELISA (ELISA). GTX42525
Anti-V5 tag antibody [SV5-Pk1] used in  (). GTX42525

Cat. No. GTX42525

Host

Mouse

Clonality

Monoclonal

Clone Name

SV5-Pk1

Isotype

IgG2a

Application

WB, ICC/IF, IHC-Fr, FACS, IP, ELISA, EMSA, RIA

Reactivity

Species independent
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/1,000-1/5,000
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA 1/1,000-1/5,000
EMSA Assay dependent
RIA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Paramyxovirus Simian-Virus 5 (SV5)

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_510174

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

V/P Antibody

Research Area

DATA IMAGES

Anti-V5 tag antibody [SV5-Pk1] used in Western Blot (WB). GTX42525

GTX42525 WB Image

WB analysis of V5 tagged protein using GTX42525 V5 tag antibody [SV5-Pk1].

Anti-V5 tag antibody [SV5-Pk1] used in ELISA (ELISA). GTX42525

GTX42525 ELISA Image

ELISA analysis of V5 tagged protein using GTX42525 V5 tag antibody [SV5-Pk1].

Anti-V5 tag antibody [SV5-Pk1] used in  (). GTX42525

GTX42525 Image

REFERENCE

REVIEW

V5 tag antibody [SV5-Pk1] Cat. No. GTX42525

Rating ( Average 4 based on 1 users reviews)
Application
Immunoprecipitation(IP) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 09-Oct-2012
Score :
Application Tested : IP
Sample : V5 fused target gene-transfected MDA-MB 468 cells
Amount used : 30 μg/well for input and 1000μg for IP(2μg/ul)
Blocking : 5% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A. 30 ug whole cell lysate/extract of transfected MDA-MB 468 cells, B. Control with 2 ug of pre-immune mouse IgG, C. Immunoprecipitation of V5 tagged recombinant protein by 2 ug of V5 tag antibody(GTX42525)
Figure Note : 2μg for IP
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399