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V5 tag antibody

Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997
Anti-V5 tag antibody used in Western Blot (WB). GTX117997
Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997

Cat No. GTX117997

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Positive Control

V5 transfected lysate

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to V5 tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618481

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

V5 tag antibody

Research Area

DATA IMAGES

Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997

GTX117997 IP Image

V5 tag recombinant protein was immunoprecipitated from HeLa transfected cell lysate by using V5 tag antibody (GTX117997). The precipitated V5 tag recomabinant was detected by GTX117997 diluted at 1:1000. A: Input B: IP by V5 tag antibody

Anti-V5 tag antibody used in Western Blot (WB). GTX117997

GTX117997 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody (GTX117997) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997

GTX117997 ICC/IF Image

V5 tag antibody detects V5 tag protein by immunofluorescent analysis.
Sample: V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: V5 tag stained by V5 tag antibody (GTX117997) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

V5 tag antibody Cat No. GTX117997

Rating ( Average 5 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 2 users reviews)
ChIP assay(ChIP assay) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-Apr-2022
Score :
Application Tested : IHC-P
Sample : human cancer injected in mouse tissue
Blocking : 5% BSA in TBST, 25°C, 0.5Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : 0.3% Triton lysis buffer/Sodium Citrate Buffer
Primary Antibody : 1:100, 4°C, > 12Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2020. PMID: 32089770
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2018. PMID: 29599809
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29731980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2019. PMID: 31681015
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2019. PMID: 31681015
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2019. PMID: 31681015
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013. PMID: 23717625
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ChIP assay
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2019. PMID: 31681015
Date : Anonymous submitted on 24-Oct-2011
Score :
Application Tested : ICC/IF
Sample : 293T cells
Blocking : 3% BSA in PBS, 25°C, 1Hr
Fixation : 2% formaldehyde in PBS
Permeabilization /Antigen retrieval : 0.1% Triton X-100/PBS
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169