ELISA: Use at a dilution of: Coating: 1:100 - 1:500Primary: 1:1000 - 1:30,000. ICC: Use at a dilution of 1/200 - 1/2000. WB: Use at a dilution of 1/1000 - 1/30000. Optimal dilutions/concentrations should be determined by the end user.
Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).
PBS, pH 7.2, sodium azide, 0.1%
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
1mg/ml(Please refer to the vial label for the specific concentration.)
Synthetic peptide: GKPIPNPLLGLDST (V5) conjugated to KLH.
Immunogen affinity purified
This antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag V5 epitope tag
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).