GeneTex
United States (US)

V5 tag antibody

Cat No. GTX29137

Host Goat
Clonality Polyclonal
Application WB, ICC/IF, ELISA, IHC
APPLICATION

Application Note

ELISA: Use at a dilution of: Coating: 1:100 - 1:500Primary: 1:1000 - 1:30,000. ICC: Use at a dilution of 1/200 - 1/2000. WB: Use at a dilution of 1/1000 - 1/30000. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2, sodium azide, 0.1%

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide: GKPIPNPLLGLDST (V5) conjugated to KLH.

Purification

Immunogen affinity purified
This antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag V5 epitope tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Research Area

Package List Price ($)
$ 329