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V5 tag antibody

Anti-V5 tag antibody used in Western Blot (WB). GTX30564

Cat No. GTX30564

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, IHC
Package
100 μg ($349)
See all V5 tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000- 1:20,000
ICC/IF 1:100-1:400
ELISA 1:1,000-1:30,000 (Detection) ; 1:100-1:500 (Coating)
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_385846

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-V5 tag antibody used in Western Blot (WB). GTX30564

GTX30564 WB Image

WB analysis of E. coli expressing a multi-tag fusion protein using GTX30564 V5 tag antibody.
Dilution : 0.04μg/ml

REFERENCE

Application Reference

Schroder HM et al. Int J Biochem Cell Biol 2013; 45 (6) : 1133-44 The tetraspanin network modulates MT1-MMP cell surface trafficking.
Application : WB
Pae SH et al. Dev Dyn 2008; 237 (4) : 962-78 Communication between integrin receptors facilitates epicardial cell adhesion and matrix organization.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349