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V5 tag antibody

Anti-V5 tag antibody used in Western Blot (WB). GTX30564

Cat. No. GTX30564

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($439)
See all V5 tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000- 1:20,000
ICC/IF 1:100-1:400
ELISA 1:1,000-1:30,000 (Detection) ; 1:100-1:500 (Coating)
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_385846

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-V5 tag antibody used in Western Blot (WB). GTX30564

GTX30564 WB Image

WB analysis of E. coli expressing a multi-tag fusion protein using GTX30564 V5 tag antibody.
Dilution : 0.04μg/ml

REFERENCE

Application Reference

Schroder HM et al. Int J Biochem Cell Biol 2013; 45 (6) : 1133-44 The tetraspanin network modulates MT1-MMP cell surface trafficking.
Application : WB
Pae SH et al. Dev Dyn 2008; 237 (4) : 962-78 Communication between integrin receptors facilitates epicardial cell adhesion and matrix organization.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 439