GeneTex
United States (US)

V5 tag antibody

V5-antibody_WB_GTX30564-1_18121411_328.jpg

Cat No. GTX30564

Reference 2
Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IP, ELISA
See all V5 tag products
APPLICATION

Application Note

For ELISA (Coating): Use at a concentration of 2-10 μg/ml. For ELISA (Primary): Use at a concentration of 0.03-1 μg/ml. For WB (Colorimetric): Use at a concentration of 0.1-1 μg/ml. For WB (ECL):Use at a concentration of 0.03-1 μg/ml. For ICC: Use at a concentratio of 0.5-5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

Antibody recognizes the V5 cleavage site.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Rabbits were immunized with V5 cleavage site (GKPIPNPLLGLDST). Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Rabbit anti-human V5 affinity purified antibodies were coupled to peptide immobilized on solid

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
V5-antibody_WB_GTX30564-1_18121411_328.jpg

GTX30564 WB Image

Whole cell lysate of E. coli expressing a multi-tag fusion protein with GTX30564 used at 0.04ug/ml and chemiluminescence with an exposure time of 3 seconds.
REFERENCE

Application Reference

Schroder HM et al. Int J Biochem Cell Biol 2013; 45 (6) : 1133-44 The tetraspanin network modulates MT1-MMP cell surface trafficking.
Application : WB
Pae SH et al. Dev Dyn 2008; 237 (4) : 962-78 Communication between integrin receptors facilitates epicardial cell adhesion and matrix organization.
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Package List Price ($)
$ 329