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VAM1 antibody [N1], N-term

Anti-VAM1 antibody [N1], N-term used in Western Blot (WB). GTX108010
Anti-VAM1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108010
Anti-VAM1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108010
Anti-VAM1 antibody [N1], N-term used in Western Blot (WB). GTX108010
Anti-VAM1 antibody [N1], N-term used in Western Blot (WB). GTX108010

Cat. No. GTX108010

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VAM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse brain , Rat brain , NT2D1

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human VAM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241437

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane palmitoylated protein 6 , PALS2 , VAM-1 , VAM1 , p55T

Cellular Localization

Membrane; Peripheral membrane protein

Background

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily (Tseng et al., 2001 [PubMed 11311936]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-VAM1 antibody [N1], N-term used in Western Blot (WB). GTX108010

GTX108010 WB Image

Sample (50 ug of whole cell lysate)
A: Rat brain
7.5% SDS PAGE
GTX108010 diluted at 1:1000

Anti-VAM1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108010

GTX108010 IHC-P Image

VAM1 antibody [N1], N-term detects VAM1 protein at on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
VAM1 antibody [N1], N-term (GTX108010) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VAM1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108010

GTX108010 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using VAM1(GTX108010) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VAM1 antibody [N1], N-term used in Western Blot (WB). GTX108010

GTX108010 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX108010 diluted at 1:1000

Anti-VAM1 antibody [N1], N-term used in Western Blot (WB). GTX108010

GTX108010 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX108010 diluted at 1:1000

REFERENCE

Application Reference

Silvia Jordans et al. Mol Neurobiol 2022; 59 (7) : 3969-3979 Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35.
Application : WB
Reactivity : Mouse
Saitoh Y et al. Histochem Cell Biol 2018; (Epub) The membrane palmitoylated protein, MPP6, is involved in myelin formation in the mouse peripheral nervous system.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169