GeneTex
United States (US)

VAMP1 antibody

GTX102127_40667_IHC_19011506_501.jpg
GTX102127_40667_IHC_M_19011506_997.jpg
GTX102127_40667_WB_19011506_307.jpg

Cat No. GTX102127

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VAMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vesicle Associated Membrane Protein 1,Spax1,Syb1,Vamp-1,Vamp1

Cellular Localization

Isoform 1: Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass type IV membrane protein , Cell junction , synapse , synaptosome , Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein (B

Background

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102127_40667_IHC_19011506_501.jpg

GTX102127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using VAMP1(GTX102127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102127_40667_IHC_M_19011506_997.jpg

GTX102127 IHC-P Image

VAMP1 antibody detects VAMP1 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
VAMP1 antibody (GTX102127) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102127_40667_WB_19011506_307.jpg

GTX102127 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
15% SDS PAGE
GTX102127 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169