GeneTex

  • Country / Location Selection

United States (US)

VAMP2 antibody [GT6311]

Anti-VAMP2 antibody [GT6311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634812
Anti-VAMP2 antibody [GT6311] used in IHC (Paraffin sections) (IHC-P). GTX634812
Anti-VAMP2 antibody [GT6311] used in Western Blot (WB). GTX634812

Cat. No. GTX634812

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT6311

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VAMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human VAMP2 Recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888483

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle associated membrane protein 2 , SYB2 , VAMP-2

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VAMP2 antibody [GT6311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634812

GTX634812 ICC/IF Image

VAMP2 antibody [GT6311] detects VAMP2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAMP2 stained by VAMP2 antibody [GT6311] (GTX634812) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VAMP2 antibody [GT6311] used in IHC (Paraffin sections) (IHC-P). GTX634812

GTX634812 IHC-P Image

VAMP2 antibody [GT6311] detects VAMP2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: VAMP2 stained by VAMP2 antibody [GT6311] (GTX634812) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VAMP2 antibody [GT6311] used in Western Blot (WB). GTX634812

GTX634812 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP2 [GT6311] (GTX634812) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jin M et al. Commun Biol 2021; 4 (1) : 961 Prediction and verification of the AD-FTLD common pathomechanism based on dynamic molecular network analysis.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for VAMP2 antibody [GT6311] (GTX634812). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations