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VAMP2 antibody

Anti-VAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133241
Anti-VAMP2 antibody used in Western Blot (WB). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241
Anti-VAMP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

Cat No. GTX133241

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse VAMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vesicle-associated membrane protein 2,Syb-2,Syb2,Vamp-2,sybII

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass type IV membrane protein , Cell junction , synapse , synaptosome

Background

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Database

Research Area

DATA IMAGES
Anti-VAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133241

GTX133241 ICC/IF Image

VAMP2 antibody detects VAMP2 protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAMP2 protein stained by VAMP2 antibody (GTX133241) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue:Fluoroshield with DAPI (GTX30920).

Anti-VAMP2 antibody used in Western Blot (WB). GTX133241

GTX133241 WB Image

Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP2 antibody (GTX133241) diluted at 1:1000.

Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VAMP2 antibody (GTX133241) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: VAMP2 protein stained by VAMP2 antibody (GTX133241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VAMP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133241

GTX133241 IHC-Fr Image

VAMP2 antibody detects VAMP2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: VAMP2 protein stained by VAMP2 antibody (GTX133241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VAMP2 antibody (GTX133241) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VAMP2 antibody (GTX133241) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169