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VAMP2 antibody

Anti-VAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241
Anti-VAMP2 antibody used in Western Blot (WB). GTX133241
Anti-VAMP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

Cat. No. GTX133241

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VAMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse VAMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886885

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle-associated membrane protein 2 , Syb-2 , Syb2 , Vamp-2 , sybII

Cellular Localization

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Single-pass type IV membrane protein , Cell junction , synapse , synaptosome

Background

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Database

Research Area

DATA IMAGES

Anti-VAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133241

GTX133241 ICC/IF Image

VAMP2 antibody detects VAMP2 protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAMP2 protein stained by VAMP2 antibody (GTX133241) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue:Fluoroshield with DAPI (GTX30920).
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VAMP2 antibody (GTX133241) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: VAMP2 protein stained by VAMP2 antibody (GTX133241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VAMP2 antibody used in Western Blot (WB). GTX133241

GTX133241 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP2 antibody (GTX133241) diluted at 1:1000.

Anti-VAMP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133241

GTX133241 IHC-Fr Image

VAMP2 antibody detects VAMP2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: VAMP2 protein stained by VAMP2 antibody (GTX133241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
VAMP2 antibody (GTX133241) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133241

GTX133241 IHC-P Image

VAMP2 antibody detects VAMP2 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
VAMP2 antibody (GTX133241) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chae CW et al. Br J Pharmacol 2020; 177 (16) : 3828-3847 High glucose-mediated PICALM and mTORC1 modulate processing of amyloid precursor protein via endosomal abnormalities.
Yokoyama T et al. Acta Histochem 2019; : 151469 Vesicular glutamate transporter 2-immunoreactive afferent nerve terminals in rat carotid sinus baroreceptors.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169