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VAMP3 antibody

Anti-VAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79273
Anti-VAMP3 antibody used in Western Blot (WB). GTX79273
Anti-VAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79273
Anti-VAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79273

Cat. No. GTX79273

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all VAMP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:10-1:100
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11163302

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle-associated membrane protein 3 , D130027G05Rik , VAMP-3 , ceb

Cellular Localization

Membrane

Background

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced.

Database

Research Area

DATA IMAGES

Anti-VAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79273

GTX79273 ICC/IF Image

ICC/IF analysis of Neuro-2a cells using GTX79273 VAMP3 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-VAMP3 antibody used in Western Blot (WB). GTX79273

GTX79273 WB Image

WB analysis of 25 ug of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates using GTX79273 VAMP3 antibody.
Dilution : 1:2000

Anti-VAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79273

GTX79273 ICC/IF Image

ICC/IF analysis of C6 cells using GTX79273 VAMP3 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-VAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79273

GTX79273 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX79273 VAMP3 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399