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VAMP5 antibody [3E5]

Anti-VAMP5 antibody [3E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632270
Anti-VAMP5 antibody [3E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632270
Anti-VAMP5 antibody [3E5] used in Western Blot (WB). GTX632270
Anti-VAMP5 antibody [3E5] used in Western Blot (WB). GTX632270

Cat. No. GTX632270

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3E5

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VAMP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human VAMP5.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888305

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle associated membrane protein 5

Cellular Localization

Cell membrane; Single-pass type IV membrane protein (Probable) , Endomembrane system; Single-pass type IV membrane protein (Probable) , Golgi apparatus , trans-Golgi network membrane; Single-pass type IV membrane protein (Probable)

Background

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in vesicle trafficking events that are associated with myogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VAMP5 antibody [3E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632270

GTX632270 ICC/IF Image

ICC/IF analysis of transfected NRK cells (V5V4-GFP) using GTX632270 VAMP5 antibody [3E5].
Red : Primary antibody

Anti-VAMP5 antibody [3E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632270

GTX632270 ICC/IF Image

ICC/IF analysis of pLEGFP-C1-hV5p transfected HeLa cells using GTX632270 VAMP5 antibody [3E5].
Red : Primary antibody

Anti-VAMP5 antibody [3E5] used in Western Blot (WB). GTX632270

GTX632270 WB Image

WB analysis of Vamp5-GST protein using GTX632270 VAMP5 antibody [3E5].

Anti-VAMP5 antibody [3E5] used in Western Blot (WB). GTX632270

GTX632270 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP5 antibody [3E5] (GTX632270) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations