APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , HepG2 membrane extract
Predict Reactivity
Mouse, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.47 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human VAPB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
VAMP associated protein B and C,ALS8,VAMP-B,VAP-B
Cellular Localization
Cell membrane; Single-pass type IV membrane protein , Endomembrane system; Single-pass type IV membrane protein
Background
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX131631 ICC/IF Image
VAPB antibody detects VAPB protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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GTX131631 WB Image
HepG2 whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX131631 WB Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX131631 ICC/IF Image
VAPB antibody detects VAPB protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for VAPB antibody (GTX131631). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VAPB antibody (GTX131631). Be the first to share your experience with this product.
