APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , HepG2 membrane extract
Predict Reactivity
Mouse, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.47 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human VAPB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886511
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
VAMP associated protein B and C , ALS8 , VAMP-B , VAP-B
Cellular Localization
Cell membrane; Single-pass type IV membrane protein , Endomembrane system; Single-pass type IV membrane protein
Background
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX131631 ICC/IF Image
VAPB antibody detects VAPB protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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GTX131631 WB Image
HepG2 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX131631 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX131631 ICC/IF Image
VAPB antibody detects VAPB protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
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REVIEW
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