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VAPB antibody

Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631
Anti-VAPB antibody used in Western Blot (WB). GTX131631
Anti-VAPB antibody used in Western Blot (WB). GTX131631
Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631

Cat No. GTX131631

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HepG2 membrane extract

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VAPB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

VAMP associated protein B and C,ALS8,VAMP-B,VAP-B

Cellular Localization

Cell membrane; Single-pass type IV membrane protein , Endomembrane system; Single-pass type IV membrane protein

Background

The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631

GTX131631 ICC/IF Image

VAPB antibody detects VAPB protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-VAPB antibody used in Western Blot (WB). GTX131631

GTX131631 WB Image

HepG2 whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VAPB antibody used in Western Blot (WB). GTX131631

GTX131631 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631

GTX131631 ICC/IF Image

VAPB antibody detects VAPB protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
There are currently no references for VAPB antibody (GTX131631). Be the first to share your publications with this product.
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf