VASP antibody [GT238]

Cat. No. GTX634448

Host	Mouse
Clonality	Monoclonal
Clone Name	GT238
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX634448 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VASP. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888452

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vasodilator stimulated phosphoprotein

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion , Cell projection , lamellipodium membrane , filopodium membrane

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]

Database

Gene ID: 7408 VASP
UniProt: P50552 VASP

Research Area

DATA IMAGES

GTX634448 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with VASP antibody [GT238] (GTX634448) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634448 IHC-P Image

VASP antibody [GT238] detects VASP protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: VASP stained by VASP antibody [GT238] (GTX634448) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody (GTX110564) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX634448 ICC/IF Image

VASP antibody [GT238] detects VASP protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VASP stained by VASP antibody [GT238] (GTX634448) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations