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VASP antibody

Orthogonal
Anti-VASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132176
Anti-VASP antibody used in IHC (Paraffin sections) (IHC-P). GTX132176
Anti-VASP antibody used in Western Blot (WB). GTX132176
Anti-VASP antibody used in Western Blot (WB). GTX132176

Cat. No. GTX132176

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VASP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , 293T(starvation16 hours , 10uM forskolin for 20 mins)

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VASP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886586

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vasodilator stimulated phosphoprotein

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion , Cell projection , lamellipodium membrane , filopodium membrane

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132176

GTX132176 ICC/IF Image

VASP antibody detects VASP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: VASP protein stained by VASP antibody (GTX132176) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-VASP antibody used in IHC (Paraffin sections) (IHC-P). GTX132176

GTX132176 IHC-P Image

VASP antibody detects VASP protein at cytoplasm in MDA-MB-231 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231 xenograft.
VASP antibody (GTX132176) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VASP antibody used in Western Blot (WB). GTX132176

GTX132176 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VASP antibody (GTX132176) diluted at 1:2000.

Anti-VASP antibody used in Western Blot (WB). GTX132176

GTX132176 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VASP antibody (GTX132176) diluted at1:1000.

REFERENCE

Application Reference

Jayakumar T et al. Int J Mol Med 2017; 39 (1) : 174-182 Nobiletin, a citrus flavonoid, activates vasodilator-stimulated phosphoprotein in human platelets through non-cyclic nucleotide-related mechanisms.
Jayakumar T. et al. Int J Mol Med 2017; 39 : 174-182 Nobiletin, a citrus flavonoid, activates vasodilator-stimulated phosphoprotein in human platelets through non-cyclic nucleotide-related mechanisms.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

VASP antibody Cat. No. GTX132176

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-VASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132176
Date : Anonymous submitted on 04-Feb-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA, 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:200, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169